CD247
Primary Info
Mutation
Mouse studies
DNA sequencing


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I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GGCTTCCTGCCTGTGAACC CAACTCGGCTGTGACGGAG 451bp chr1:167487454-167487904
2 TCAGTCCTAGTGCCACCCTG CTGGTCCTTGCTTTGCTCCT 322bp chr1:167409766-167410087
3 GTTAGTTGCCAAGGAGCGGA TCGCAGAGGTGATAGCTAGG 290bp chr1:167408463-167408752
4 CTCCAGATGGACCAGTTACCA TCTGGTTGCAGAGTGAGCTG 328bp chr1:167407711-167408038
5 TGAGCCATGTAGTGCCATGAG CCCTTCCTCCAACTTTCCAAGGT 270bp chr1:167404588-167404857
6 CTGCCACTGCTGCCAACTT AGGTTCGCCTTCACCTCTGA 303bp chr1:167403117-167403419
7 GCCAGGCCTCACCTTACTCT GGACTTATTGGCAGTGGCGT 401bp chr1:167402094-167402494
8a GCTGCAGAGACACCTGTTGG CATGTGCTAGCATCTGCGCT 525bp chr1:167400549-167401073

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GGCTTCCTGCCTGTGAACC CAACTCGGCTGTGACGGAG 451bp chr1:167487454-167487904
2 TCAGTCCTAGTGCCACCCTG CTGGTCCTTGCTTTGCTCCT 322bp chr1:167409766-167410087
3 GTTAGTTGCCAAGGAGCGGA TCGCAGAGGTGATAGCTAGG 290bp chr1:167408463-167408752
4 CTCCAGATGGACCAGTTACCA TCTGGTTGCAGAGTGAGCTG 328bp chr1:167407711-167408038
5 TGAGCCATGTAGTGCCATGAG CCCTTCCTCCAACTTTCCAAGGT 270bp chr1:167404588-167404857
6 CTGCCACTGCTGCCAACTT AGGTTCGCCTTCACCTCTGA 303bp chr1:167403117-167403419
7 GCCAGGCCTCACCTTACTCT GGACTTATTGGCAGTGGCGT 401bp chr1:167402094-167402494
8a GCTGCAGAGACACCTGTTGG CATGTGCTAGCATCTGCGCT 525bp chr1:167400549-167401073

IV. Ethanol precipitation

V. Sequencing analysis