ICOS
Primary Info
Mutation
Mouse studies
DNA sequencing


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I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TGCCTTTCATATAGCCATTG AGTCATTTTGCCTTTCATCTTT 572bp chr2:204801355+204801926
2 GCAACAGAGATGACTTTATGCATTGA CTGCATCTAAGTGAACTCCAA 567bp chr2:204820248+204820814
3 AAGCCCTTTCTATAAACCACATT TGTCTCCCTGTTGGTCAAA chr2:?-204821602
4 GCCAGGAACCTAAGTCACATTATC AATTGCCTTGATACTATGGTGG 480bp chr2:204822394+204822873
5a GGCAATGGAGAGGGGAAAGCT GGCTGTCAAGCAGTCATGTAGA 747bp chr2:204824227+204824973

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TGCCTTTCATATAGCCATTG AGTCATTTTGCCTTTCATCTTT 572bp chr2:204801355+204801926
2 GCAACAGAGATGACTTTATGCATTGA CTGCATCTAAGTGAACTCCAA 567bp chr2:204820248+204820814
3 AAGCCCTTTCTATAAACCACATT TGTCTCCCTGTTGGTCAAA chr2:?-204821602
4 GGAGAAGAAAAGATGACCAAGC TGTGAAGAAACCTCTTCCCTTC 176bp chr2:204822477+204822652
5a GGCAATGGAGAGGGGAAAGCT GGCTGTCAAGCAGTCATGTAGA 747bp chr2:204824227+204824973

IV. Ethanol precipitation

V. Sequencing analysis