IGHM
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1a ACTCAGAACGCCACTCAG TGAGGTGGCTGCGTACTT 293bp chr14:106322117-106322409
1b CTTCCCATCAGTCCTGAG ACGAAGACGCTCACTTTG 276bp chr14:106321884-106322159
2 AAAGAACGTGCCTCTTCCAG CCAAGGCTCAGGGAGTAGATG 549bp chr14:106321482-106322030
3a GGCTCGGCAGAGGCAGTT ACCCACGGCGCTGAAAGT 305bp chr14:106321131-106321435
3b ATGACAGCGTGACCATCT GGGGTGCTTTCCACCATG 290bp chr14:106320945-106321234
4a CATGGTGGAAAGCACCCC TGGCAGCAAGTAGACATC 158bp chr14:106320805-106320962
4b GATGTCTACTTGCTGCCA GGTTTACCGGTGGACTTG 320bp chr14:106320503-106320822
4c TGTCTACTTGCTGCCACCAG CAGGGAATTCAGGGGTTTCT 655bp chr14:106320166-106320820
5-6 AAGGGTGGGCCTAGAGGAT CGGTCGAGAAGGAAGCCTGT 541bp chr14:106318144-106318684

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1a ACTCAGAACGCCACTCAG TGAGGTGGCTGCGTACTT 293bp chr14:106322117-106322409
1b CTTCCCATCAGTCCTGAG ACGAAGACGCTCACTTTG 276bp chr14:106321884-106322159
2 AAAGAACGTGCCTCTTCCAG CCAAGGCTCAGGGAGTAGATG 549bp chr14:106321482-106322030
3a GGCTCGGCAGAGGCAGTT ACCCACGGCGCTGAAAGT 305bp chr14:106321131-106321435
3b ATGACAGCGTGACCATCT GGGGTGCTTTCCACCATG 290bp chr14:106320945-106321234
4a CATGGTGGAAAGCACCCC TGGCAGCAAGTAGACATC 158bp chr14:106320805-106320962
4b GATGTCTACTTGCTGCCA GGTTTACCGGTGGACTTG 320bp chr14:106320503-106320822
4c TGTCTACTTGCTGCCACCAG CAGGGAATTCAGGGGTTTCT 655bp chr14:106320166-106320820
5-6 AAGGGTGGGCCTAGAGGAT CGGTCGAGAAGGAAGCCTGT 541bp chr14:106318144-106318684

IV. Ethanol precipitation

V. Sequencing analysis