IL2RG
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AGCTGTATATGTGCCCACAG ACTCCACTTTTCAATTCTGCC 730bp chrX:70331155-70331884
2-3 TCCTTCTGACCATCATTTCTC AGTATCCCTGGTCTCTTGAC 641bp chrX:70330316-70330956
4 GAGATCCACCTCTACCAAAC AATAGGGGGGAAAGGAGTTG 605bp chrX:70329843-70330447
5 AGGAAGCCATTAAGGGGATC TTGCAGGCTCTCTAGGAATG 638bp chrX:70328798-70329435
6-7 AGAGTATGTCTTTAGCGCGG AGTCGTTCACTGTAGTCTGG 932bp chrX:70327710-70328641
8 CAGTGGTTGCAGACATCATG AAACCCTGGAATGAGTGCAG 791bp chrX:70327079-70327869

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AGCTGTATATGTGCCCACAG ACTCCACTTTTCAATTCTGCC 730bp chrX:70331155-70331884
2-3 TCCTTCTGACCATCATTTCTC AGTATCCCTGGTCTCTTGAC 641bp chrX:70330316-70330956
4 GAGATCCACCTCTACCAAAC AATAGGGGGGAAAGGAGTTG 605bp chrX:70329843-70330447
5 AGGAAGCCATTAAGGGGATC TTGCAGGCTCTCTAGGAATG 638bp chrX:70328798-70329435
6-7 AGAGTATGTCTTTAGCGCGG AGTCGTTCACTGTAGTCTGG 932bp chrX:70327710-70328641
8 CAGTGGTTGCAGACATCATG AAACCCTGGAATGAGTGCAG 791bp chrX:70327079-70327869

IV. Ethanol precipitation

V. Sequencing analysis