• Sample Genome 1µL+Sample Buffer 9 µL
• 95℃, 3min
• 4℃ or on ice
• Making PreMix
• Reaction Buffer 9 µL+Enzyme Mix 1 µL / Sample genome 1 µL
• Add PreMix 9 µL to each 1 sample (each tube)
• 30℃, 90 min
• 65℃, 10 min
• 4℃,∞

For Exon(s):3,4,5,6,7,8,9,10,11,12a

For Exon(s):2

PCR Clean-Up Kit ExoSAP-IT®
At first, ExoSAP-IT is diluted by 5% using sterilized distilled water
(ExoSAP-IT 0.2 µL + Sterilized distilled water 3.8 µL / 1 sample ).