JAK3
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2-3 TCCAGCACTCCTTTCCATGC CCTCGAAATGCAAGGAGATG 802bp chr19:17954524-17955325
4-5 GAGGCCTCCGCAGAATCTG CATAGGAACACCCTGAAAGC 683bp chr19:17953716-17954398
6 TAAAGCCTGGGTTTGTGTGTG TCATTAAGCCTCGCCCACTTC 503bp chr19:17952997-17953499
7-8 GTGTTGAGAGGTTGGAGTTG TTCACTGAGCGCTGACTGTG 676bp chr19:17952053-17952728
9 GATCCCAGGGCTACAGAGGT GGGGTGTCTTTTCCCTCCTA 327bp chr19:17950921-17951247
10 AAAGGAAGAAGGACTGAGAAGG ACCCACACAGGAGCTGTCAC 335bp chr19:17950207-17950541
11 GTCTCAACAGCAGCAGCAAC GTGAGGGGACAGCAGAAGAG 497bp chr19:17948880-17949376
12 GGGCCAGCAGCTATGTCTC TTTCTCTGCATCCACGACC 284bp chr19:17948672-17948955
13 ATCATGGTAGTGCTGTGTGC GCTGTCATATAGCGGCTCAG 329bp chr19:17947848-17948176
14 TACAGATACAGACAGGGCTC CGCCCTGACATCCAAGAATG 532bp chr19:17946550-17947081
15 GAGAAGGGGAGGGACATAGC AATCCCCAACCCAATAGACC 666bp chr19:17945555-17946220
16-17 CCTTCATCAAGCTGAGTGACC CTCCAACCTCACCAGACACA 675bp chr19:17945271-17945945
18-19 CATAATGCACAGAGAGGGTC TTCCCAGCCTACCTAAAGTG 620bp chr19:17943231-17943850
20-21 AACTGAGGTCGAGAGGGACAC CTAAGGCTGGGGAGCAAAG 774bp chr19:17941935-17942708
22 GGGCTGGAGAATCCACCTAT AAGGGGTCAGAAAGGTGAGG 447bp chr19:17941150-17941596
23 ACCTGGTCCCAGCATCATAG TGACTTGCCACACAGTGAGAG 460bp chr19:17940751-17941210
24 TACGCTGAATGGGAGTTGTG CTTAAGGGGTTGGGAAGATG 529bp chr19:17937261-17937789

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2-3 TCCAGCACTCCTTTCCATGC CCTCGAAATGCAAGGAGATG 802bp chr19:17954524-17955325
4-5 GAGGCCTCCGCAGAATCTG CATAGGAACACCCTGAAAGC 683bp chr19:17953716-17954398
6 TAAAGCCTGGGTTTGTGTGTG TCATTAAGCCTCGCCCACTTC 503bp chr19:17952997-17953499
7-8 GTGTTGAGAGGTTGGAGTTG TTCACTGAGCGCTGACTGTG 676bp chr19:17952053-17952728
9 GATCCCAGGGCTACAGAGGT GGGGTGTCTTTTCCCTCCTA 327bp chr19:17950921-17951247
10 AAAGGAAGAAGGACTGAGAAGG ACCCACACAGGAGCTGTCAC 335bp chr19:17950207-17950541
11 GTCTCAACAGCAGCAGCAAC GTGAGGGGACAGCAGAAGAG 497bp chr19:17948880-17949376
12 GGGCCAGCAGCTATGTCTC TTTCTCTGCATCCACGACC 284bp chr19:17948672-17948955
13 ATCATGGTAGTGCTGTGTGC GCTGTCATATAGCGGCTCAG 329bp chr19:17947848-17948176
14 TACAGATACAGACAGGGCTC CGCCCTGACATCCAAGAATG 532bp chr19:17946550-17947081
15 GAGAAGGGGAGGGACATAGC AATCCCCAACCCAATAGACC 666bp chr19:17945555-17946220
16-17 CCTTCATCAAGCTGAGTGACC CTCCAACCTCACCAGACACA 675bp chr19:17945271-17945945
18-19 CATAATGCACAGAGAGGGTC TTCCCAGCCTACCTAAAGTG 620bp chr19:17943231-17943850
20-21 AACTGAGGTCGAGAGGGACAC CTAAGGCTGGGGAGCAAAG 774bp chr19:17941935-17942708
22 GGGCTGGAGAATCCACCTAT AAGGGGTCAGAAAGGTGAGG 447bp chr19:17941150-17941596
23 ACCTGGTCCCAGCATCATAG TGACTTGCCACACAGTGAGAG 460bp chr19:17940751-17941210
24 TACGCTGAATGGGAGTTGTG CTTAAGGGGTTGGGAAGATG 529bp chr19:17937261-17937789

IV. Ethanol precipitation

V. Sequencing analysis