PSTPIP1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GAGCTCCAGCCTGCCTCT GGCCACCTCCATGACAATTA 577bp chr15:77287523+77288099
2 TCACTTATGTACCTGTCACCTCTG TCATCAGTGACTTGCCACCTC 493bp chr15:77310279+77310771
3 CCAGAGGTGGCAAGTCACTG CAAGGAGCAATCCAGACCTAC 255bp chr15:77310748+77311002
4-5 GCAGCCTGGACTGTAGGTTC GGCTCTACCAGGAAGGTCAGC 478bp chr15:77317560+77318037
6 CTCCAGAGCCAGGAGAGGTG GAGGTCAGCCTGTTCCAAATG 284bp chr15:77320081+77320364
7 CGCCTTCTCTATCTCCTTCCG CAGATGCAGATCTTGCCTGG 373bp chr15:77320722+77321094
8 TAGTTGGCTCCTGAATGTTCC CGGCACATATCTGGAATGAGAG 312bp chr15:77321746+77322057
9 GGGACCTGCTGGAGTACAGG AGTAGGGATGGGCGTCTCAC 213bp chr15:77322773+77322985
10 GTTGCAGGCAGGCATTCTTC GAGGACCAGGTGCTTGTTGAC 592bp chr15:77323126+77323717
11 CAGAGTTCAGGCCCACAGAG TTAGCTTTGCCTGAGAGGAGG 350bp chr15:77324572+77324921
12 AGGGCCAGATTGGGAATGTAG CTGGGCACCAGATACAGACAC 311bp chr15:77325107+77325417
13 AGGAGGAGCCACCAGGACTAC CCTCTTCCTTCTGCTGTCCAC 407bp chr15:77327643+77328049
14 TGTGGACAGCAGAAGGAAGAG AAGGCACTGGATGGGAGAG 325bp chr15:77328028+77328352
15 AGGCCCTTCCAACGTCAT CTCCTCTGAACTGAGCACTCG 395bp chr15:77329346+77329740

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 GAGCTCCAGCCTGCCTCT GGCCACCTCCATGACAATTA 577bp chr15:77287523+77288099
2 TCACTTATGTACCTGTCACCTCTG TCATCAGTGACTTGCCACCTC 493bp chr15:77310279+77310771
3 CCAGAGGTGGCAAGTCACTG CAAGGAGCAATCCAGACCTAC 255bp chr15:77310748+77311002
4-5 GCAGCCTGGACTGTAGGTTC GGCTCTACCAGGAAGGTCAGC 478bp chr15:77317560+77318037
6 CTCCAGAGCCAGGAGAGGTG GAGGTCAGCCTGTTCCAAATG 284bp chr15:77320081+77320364
7 CGCCTTCTCTATCTCCTTCCG CAGATGCAGATCTTGCCTGG 373bp chr15:77320722+77321094
8 TAGTTGGCTCCTGAATGTTCC CGGCACATATCTGGAATGAGAG 312bp chr15:77321746+77322057
9 GGGACCTGCTGGAGTACAGG AGTAGGGATGGGCGTCTCAC 213bp chr15:77322773+77322985
10 GTTGCAGGCAGGCATTCTTC GAGGACCAGGTGCTTGTTGAC 592bp chr15:77323126+77323717
11 CAGAGTTCAGGCCCACAGAG TTAGCTTTGCCTGAGAGGAGG 350bp chr15:77324572+77324921
12 AGGGCCAGATTGGGAATGTAG CTGGGCACCAGATACAGACAC 311bp chr15:77325107+77325417
13 AGGAGGAGCCACCAGGACTAC CCTCTTCCTTCTGCTGTCCAC 407bp chr15:77327643+77328049
14 TGTGGACAGCAGAAGGAAGAG AAGGCACTGGATGGGAGAG 325bp chr15:77328028+77328352
15 AGGCCCTTCCAACGTCAT CTCCTCTGAACTGAGCACTCG 395bp chr15:77329346+77329740

IV. Ethanol precipitation

V. Sequencing analysis