RAG1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2a GAGGAAATAACTCTATGATCAGCACC CACGTTCCTCGGGAAGTAAAC 788bp chr11:36594667+36595454
2b AGTTCTGCCATAACTGCTGG ACTGACTGCAGCTGAGGAAG 930bp chr11:36595375+36596304
2c GTGAAGTCCGTGTGCATGAC AGGTTCTCAGCATGGCTTCT 983bp chr11:36596142+36597124
2d AAAACTTGTGCGGGAAGTGG GGTGTTTCAGGACATCTTCC 845bp chr11:36596990+36597834
2e TTATTGAGAGGGATGGCTCC ATGACAGCAGATGACCTCCT 985bp chr11:36597700+36598684

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2a GAGGAAATAACTCTATGATCAGCACC CACGTTCCTCGGGAAGTAAAC 788bp chr11:36594667+36595454
2b AGTTCTGCCATAACTGCTGG ACTGACTGCAGCTGAGGAAG 930bp chr11:36595375+36596304
2c TCATGCAGGGAAAGGGCTCT AGGTTCTCAGCATGGCTTCT 901bp chr11:36596224+36597124
2d AAAACTTGTGCGGGAAGTGG GGTGTTTCAGGACATCTTCC 845bp chr11:36596990+36597834
2e TTATTGAGAGGGATGGCTCC ATGACAGCAGATGACCTCCT 985bp chr11:36597700+36598684

IV. Ethanol precipitation

V. Sequencing analysis