CSF2RA
Primary Info
Mutation
Mouse studies
DNA sequencing


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I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
3 CACCATCCTGGGTTTGTTTC ATGCAGATGGACCCAGGTATC 299bp chrX:1401467+1401765; chrY:1351467+1351765
4 AGCTTGCAGCTTGCAGTGAC GCCCTGTTGCTTTTAACTCAG 361bp chrX:1404522+1404882; chrY:1354522+1354882
5 CGGGTGTCTGTAATCCGAGC CAACGGGATGTACTTTGATGC 368bp chrX:1407243+1407610; chrY:1357243+1357610
6 AGCTCAGGGATCCGTTTACAG CACTTGAACCCTGGAGGGAG 578bp chrX:1407547+1408124; chrY:1357547+1358124
7 AAGTGATCTGCCTGCCTCAAC CACGTGGCCTCAGTTACACAG chrX:?-1409484; chrY:?-1359484
8 CAGGACCTACTCCACCTCCAC GCTACCCGAAGTGCGTTCAC 444bp chrX:1413059+1413502; chrY:1363059+1363502
9 GGTTTGTGGAGGGAGACCTG ACCGTAGGGTTACGCTGGAG 316bp chrX:1414175+1414490; chrY:1364175+1364490
10 CCAAGTGCATTCAGAGTGGTAG ACACTACTCCCAGCGACGTG 406bp chrX:1419237+1419642; chrY:1369237+1369642
11 GCAGGTTGCTTTCATCTCAG CCAAGGCTGTGTTTCTCTTTC 393bp chrX:1422629+1423021; chrY:1372629+1373021
12 TGCATAGTTGAGCGCAGACAC CCATCTGGTGGAGAAGCAAG 300bp chrX:1424225+1424524; chrY:1374225+1374524
13a AGAAAATAAACACAGCCCACTGAG CCAAGTAGCTGGGATGATGG 457bp chrX:1428219+1428675; chrY:1378219+1378675

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
3 CACCATCCTGGGTTTGTTTC ATGCAGATGGACCCAGGTATC 299bp chrX:1401467+1401765; chrY:1351467+1351765
4 GAGGAAATTCTGAACCCAGTG GCCCTGTTGCTTTTAACTCAG 247bp chrX:1404636+1404882; chrY:1354636+1354882
5 CGGGTGTCTGTAATCCGAGC CAACGGGATGTACTTTGATGC 368bp chrX:1407243+1407610; chrY:1357243+1357610
6 AGCTCAGGGATCCGTTTACAG CACTTGAACCCTGGAGGGAG 578bp chrX:1407547+1408124; chrY:1357547+1358124
7 TTTGTTTTGTTTTCCTGATTGC CACGTGGCCTCAGTTACACAG 303bp chrX:1409182+1409484; chrY:1359182+1359484
8 CAGGACCTACTCCACCTCCAC GCTACCCGAAGTGCGTTCAC 444bp chrX:1413059+1413502; chrY:1363059+1363502
9 GGTTTGTGGAGGGAGACCTG ACCGTAGGGTTACGCTGGAG 316bp chrX:1414175+1414490; chrY:1364175+1364490
10 CCAAGTGCATTCAGAGTGGTAG ACACTACTCCCAGCGACGTG 406bp chrX:1419237+1419642; chrY:1369237+1369642
11 GCAGGTTGCTTTCATCTCAG CCAAGGCTGTGTTTCTCTTTC 393bp chrX:1422629+1423021; chrY:1372629+1373021
12 TGCATAGTTGAGCGCAGACAC CCATCTGGTGGAGAAGCAAG 300bp chrX:1424225+1424524; chrY:1374225+1374524
13a AGAAAATAAACACAGCCCACTGAG CCAAGTAGCTGGGATGATGG 457bp chrX:1428219+1428675; chrY:1378219+1378675

IV. Ethanol precipitation

V. Sequencing analysis