CD19
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CCTGCCTGCCCCAGCATC GGACACCCAGCCACAACTCAAG 209bp chr16:28943265+28943473
2 TACCCTCTCTGAGCCTCCAT GACAGGAAGCAGCTCATACTGAAG 621bp chr16:28943488+28944108
3 CTTCAGTATGAGCTGCTTCCTGTC AGCTCTAGGCTCAGCAATGACTTA 600bp chr16:28944085+28944684
4 CAGAGACAGAGGGGAGGGGAAA GGAGTCAGTGTTCTGTGTGGAAGT 496bp chr16:28944475+28944970
5 CAGACTTGCGGTTCCTTGAGAT GAAGACTGGGGAGAACAGGA 335bp chr16:28946682+28947016
6 CAATTTGAACTCCTGTCTCCT AGTAGAGACGGAGCAAAGAAC 266bp chr16:28947358+28947623
7 GGCCTGGACAGAATCTTGAGTG CACAGCGGATTGGAGGGATGA 254bp chr16:28947754+28948007
8 GACCCAGAGCCTGAAGTCGT ATGGGGATGGGGAACCGAGT 456bp chr16:28948122+28948577
9-10 GTCTGTGGCCCGAATAGTG GGTCAGCTCTTCATCCTCGTTCT 469bp chr16:28948511+28948979
11-12 AAGACTCCTTCTCCAACGGTAACTT GGGTCAGGGTCAGGTCAAAGTT 413bp chr16:28948827+28949239
13-14 CCTCTGCCCCTCAGTCTTCCTC CTGCCATCCGTGCCTATCT 445bp chr16:28949914+28950358

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CCTGCCTGCCCCAGCATC GGACACCCAGCCACAACTCAAG 209bp chr16:28943265+28943473
2 TACCCTCTCTGAGCCTCCAT GACAGGAAGCAGCTCATACTGAAG 621bp chr16:28943488+28944108
3 CTTCAGTATGAGCTGCTTCCTGTC AGCTCTAGGCTCAGCAATGACTTA 600bp chr16:28944085+28944684
4 CAGAGACAGAGGGGAGGGGAAA GGAGTCAGTGTTCTGTGTGGAAGT 496bp chr16:28944475+28944970
5 CAGACTTGCGGTTCCTTGAGAT GGGAAGATGGAGTAGGGTTT 242bp chr16:28946682+28946923
6 CAATTTGAACTCCTGTCTCCT AGTAGAGACGGAGCAAAGAAC 266bp chr16:28947358+28947623
7 GGCCTGGACAGAATCTTGAGTG CACAGCGGATTGGAGGGATGA 254bp chr16:28947754+28948007
8 GACCCAGAGCCTGAAGTCGT ATGGGGATGGGGAACCGAGT 456bp chr16:28948122+28948577
9-10 GTCTGTGGCCCGAATAGTG CAGCTCTTCATCCTCGTTCTCATAA 466bp chr16:28948511+28948976
11-12 AAGACTCCTTCTCCAACGGTAACTT GGGTCAGGGTCAGGTCAAAGTT 413bp chr16:28948827+28949239
13-14 CCTCTGCCCCTCAGTCTTCCTC CTGCCATCCGTGCCTATCT 445bp chr16:28949914+28950358

IV. Ethanol precipitation

V. Sequencing analysis