CD3D
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 ATTGTCAATAGGGACGCTAAAGT TCACTCCCATCAGTAATGTCC 405bp chr11:118213207-118213611
2 TTGATGGAAGCCATAAACTGTC GGGTTCACCATATCCCTCTCTAG 432bp chr11:118211003-118211434
3 TGGAGGAAGGTGTGGCAAG GGTGCATAAGCTCACTGGTACAC 377bp chr11:118210406-118210782
4-5 GCTGGTGGTAGGTTTAGTCTCA GATCCCAAGCAAGTCAGGAC 668bp chr11:118209724-118210391

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 ATTGTCAATAGGGACGCTAAAGT TCACTCCCATCAGTAATGTCC 405bp chr11:118213207-118213611
2 TTGATGGAAGCCATAAACTGTC GGGTTCACCATATCCCTCTCTAG 432bp chr11:118211003-118211434
3 TGGAGGAAGGTGTGGCAAG ACACAAACACACTCTCATGCTC 330bp chr11:118210453-118210782
4-5 GCTGGTGGTAGGTTTAGTCTCA GATCCCAAGCAAGTCAGGAC 668bp chr11:118209724-118210391

IV. Ethanol precipitation

V. Sequencing analysis