CYBB
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TTAATTGTGACTGGATCATTATAGACC GGAATTTGGAAGAATGGACTCAG 439bp chrX:37639105+37639543
2 ACTGTTGAGGAAAGAGCTGGC ATGGAGGGAGTGAGGCTAATG 395bp chrX:37641178+37641572
3 CCTCATGCTAAGAACCTTGG TTGATGGCCTTTGAAAATTAGAGGAACTTAG 247bp chrX:37642656+37642902
4 TGATACAGTTTGCAGGGTGGTC TTGAAGTGGCAGAAAGTGTGG 373bp chrX:37651109+37651481
5 GCTGTCCCAGAAACCCAGCTTAC GCAAAGGAGAGGTCTTCACTCAC 310bp chrX:37652840+37653149
6 CTGTCTGTGAGGGATGATTAGG GCAGACAGGCTAACCACACAC 481bp chrX:37655024+37655504
7 TCTATTCAGAGGGAGCAATAAGC TTTTAGCTGTTCCATGACATTTG 402bp chrX:37658063+37658464
8 GCCTACAAAGATTAACTTGGCTTC GAGGAGTTGGGAAGACTTGTCC 267bp chrX:37660406+37660672
9 CCATATGACACTAAAAAGGCAAG CCTGAACTCATATACGTTGG 363bp chrX:37663062+37663424
10 CCAATTTGAATTAACATGTTGCAC CAACCTTAGCCCTGAAAGCAC 355bp chrX:37664154+37664508
11 GTGGAATTCCACATGGTAAT GTCACTATGGAAGGACCTGAG chrX:37665568-?
12 AGGGTGCCTTGGTTAGAATAGC ATCTGGAATCTTCTGTTGGGC 374bp chrX:37668720+37669093
13 TACCTGCTTTGTAGACATCTC GAGCATTTGGCAGCACAACC 239bp chrX:37669981+37670219

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 TTAATTGTGACTGGATCATTATAGACC GGAATTTGGAAGAATGGACTCAG 439bp chrX:37639105+37639543
2 ACTGTTGAGGAAAGAGCTGGC ATGGAGGGAGTGAGGCTAATG 395bp chrX:37641178+37641572
3 CCTCATGCTAAGAACCTTGG TTGATGGCCTTTGAAAATTAGAGGAACTTAG 247bp chrX:37642656+37642902
4 TGATACAGTTTGCAGGGTGGTC TTGAAGTGGCAGAAAGTGTGG 373bp chrX:37651109+37651481
5 GCTGTCCCAGAAACCCAGCTTAC GCAAAGGAGAGGTCTTCACTCAC 310bp chrX:37652840+37653149
6 TGTGTGTGTTTATATTTTACAGAACC GCAGACAGGCTAACCACACAC 323bp chrX:37655182+37655504
7 TCTATTCAGAGGGAGCAATAAGC TTTTAGCTGTTCCATGACATTTG 402bp chrX:37658063+37658464
8 GCCTACAAAGATTAACTTGGCTTC GAGGAGTTGGGAAGACTTGTCC 267bp chrX:37660406+37660672
9 CCATATGACACTAAAAAGGCAAG CCTGAACTCATATACGTTGG 363bp chrX:37663062+37663424
10 CCAATTTGAATTAACATGTTGCAC CAACCTTAGCCCTGAAAGCAC 355bp chrX:37664154+37664508
11 GTGGAATTCCACATGGTAAT GTCACTATGGAAGGACCTGAG chrX:37665568-?
12 AGGGTGCCTTGGTTAGAATAGC ATCTGGAATCTTCTGTTGGGC 374bp chrX:37668720+37669093
13 TACCTGCTTTGTAGACATCTC GAGCATTTGGCAGCACAACC 239bp chrX:37669981+37670219

IV. Ethanol precipitation

V. Sequencing analysis