ROBLD3
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CTGCTGCTGTCCTTTCACTCC CTCCCTTCCTGGTGATCCTTC 457bp chr1:156024387+156024843
2 CCAGAGCCTTGCTGGATGTG AATCTTCCGGAGATCCTGTCC 377bp chr1:156024951+156025327
3 GCTGAACTTCCTGCTGTGCC CCACACCAGAAATGGACTGAG 343bp chr1:156027638+156027980
4 CTCAGTCCATTTCTGGTGTGG TGGGTAGAAGTCCATCCTTGC 442bp chr1:156027960+156028401

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CTGCTGCTGTCCTTTCACTCC CTCCCTTCCTGGTGATCCTTC 457bp chr1:156024387+156024843
2 CCAGAGCCTTGCTGGATGTG AATCTTCCGGAGATCCTGTCC 377bp chr1:156024951+156025327
3 GCTGAACTTCCTGCTGTGCC CCACACCAGAAATGGACTGAG 343bp chr1:156027638+156027980
4 CTCAGTCCATTTCTGGTGTGG TGGGTAGAAGTCCATCCTTGC 442bp chr1:156027960+156028401

IV. Ethanol precipitation

V. Sequencing analysis