IFNGR2
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CCCGCTGCTGCTCGGGAAGA ACCCCGCAAGACCCCCACCT 417bp chr21:34775713+34776129
2 TGTGCTGCCTGTACCAGTAGG ACAAGCTCCAAGAACGATGTG 479bp chr21:34786987+34787465
3 GAACAAAAGCTCTGGGGAAAC GAGTCGATACCGCAAGTGAAG 348bp chr21:34793712+34794059
4 GCGACAGAGCAAAACTGTCTC GCCCATGTTAAAGGTTTCTTTC 420bp chr21:34799004+34799423
5 TCTTTGGTTGTCGTGTTCACAG ACCACCCATGACCTCATAAGC 356bp chr21:34804380+34804735
6 CTCTTTAAGCAGCATGGATGG GAGATGTGTGCACACGTACCT 265bp chr21:34804933+34805197
7 AAAGGTCTGGTATACTGAACTGGTAAAC TGCTAACAAGCAATTACATGCC 478bp chr21:34809075+34809552

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 CCCGCTGCTGCTCGGGAAGA ACCCCGCAAGACCCCCACCT 417bp chr21:34775713+34776129
2 TGTGCTGCCTGTACCAGTAGG ACAAGCTCCAAGAACGATGTG 479bp chr21:34786987+34787465
3 GAACAAAAGCTCTGGGGAAAC GAGTCGATACCGCAAGTGAAG 348bp chr21:34793712+34794059
4 GCGACAGAGCAAAACTGTCTC GCCCATGTTAAAGGTTTCTTTC 420bp chr21:34799004+34799423
5 TCTTTGGTTGTCGTGTTCACAG ACCACCCATGACCTCATAAGC 356bp chr21:34804380+34804735
6 CTCTTTAAGCAGCATGGATGG GAGATGTGTGCACACGTACCT 265bp chr21:34804933+34805197
7 AAAGGTCTGGTATACTGAACTGGTAAAC CCATGAGACCTGCTGTCTCT 348bp chr21:34809075+34809422

IV. Ethanol precipitation

V. Sequencing analysis