STAT1
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
3 ACAACCTGATGCTAGGAAGGC GCCATTGATGGAATTCATCTTC 315bp chr2:191874497-191874811
4 AAAGCAATGTCAGTTTCAAAAGAAG TGTGTGCTCAATTGTATTTGCTG 385bp chr2:191873600-191873984
5 GGTACCTGCACTTAGAAAGTTAAAGC TCTGATTCTCCCACTTCTTGG 443bp chr2:191872153-191872595
6 CATGTCAGAAGATCTGGAAGGC AAAAGACTGAACTCAGTTACGAGG 300bp chr2:191865697-191865996
7 CATAGAAGCAATACTCTGTTGCC GGTTATCAAGGAAGAATTCAGAGTC 284bp chr2:191864226-191864509
8 TTTGACCTGTCACTAGGCAGC TTTCCTCTCTTCTAAACTTTGAGTCC 287bp chr2:191862862-191863148
9 GCCCCAGTTGAGAATGAAATG CAGGGTAAGCAGAAGCTGGAC 401bp chr2:191862439-191862839
10 ATTCCCTTTAATCCAGGCTGC TGAATTAACGGTAAAATGTTCCTC 298bp chr2:191859716-191860013
11 TTCAGGAAAATCATTGTGATTGC TTTCCTCAAAAGCACCCTATATAAC 268bp chr2:191855854-191856121
12 TAAGCGGTTCCACAGCTAGG GTGGAGGAATCTGTGCTTGAG 344bp chr2:191854262-191854605
13-14 TCATCATTCACTTACACTCTTATGCTC ACAGGAAGACACCAGCCAC 459bp chr2:191851440-191851898
15 GGTTGTGATTCCAAGAGATCC ACCAACTGACCTGTCCTTGC 347bp chr2:191850274-191850620
16 TGAGTCTTCAGACTTGCCACTG AATACCTCCAGAACAAACACTGAG 309bp chr2:191848940-191849248
17 AGGCTAAGCAGTTATCTGAAGGTG ATGCAGCAGTGAGAGCGTG 234bp chr2:191848303-191848536
18 CTCTTCCCCTACTGTGAAAGCA CTCATCATGTCAGGGACTTCCT 646bp chr2:191846734-191847379
19 CAAGAGCCATCCGTCCAAC GCCAGAGAAGAACACGCC 243bp chr2:191845227-191845469
20 CTGTGCTGAATGGGACAGTTC AATACTGAAGCTGGACTCAGGC 303bp chr2:191844415-191844717
21 ACATTCCAGCCATTTTCTTGG ATGTCCCAAACGCACTATCTG 338bp chr2:191843483-191843820
22 TGTCCTCTTTCATTTTGGGATG CTGCATGGGTGGAGTTTCAG 326bp chr2:191841483-191841808
23 GGAGGCTAAGCTGTCTAGAAACAC GTTCAGCTGTGATGGCGATAG 314bp chr2:191840413-191840726
24 TCTGGCTATTGCTTATCACATCC GCTCATCTGAGCACTGCACTC 482bp chr2:191839416-191839897
25a GACAAGCAATGTTTAATGTATGAGTC CCCACAGAAATTTACCGTTCC 523bp chr2:191835000-191835522

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
3 ACAACCTGATGCTAGGAAGGC GCCATTGATGGAATTCATCTTC 315bp chr2:191874497-191874811
4 AAAGCAATGTCAGTTTCAAAAGAAG TGTGTGCTCAATTGTATTTGCTG 385bp chr2:191873600-191873984
5 GGTACCTGCACTTAGAAAGTTAAAGC TTGCTTTGACATGGGCCCTA 394bp chr2:191872202-191872595
6 CATGTCAGAAGATCTGGAAGGC AAAAGACTGAACTCAGTTACGAGG 300bp chr2:191865697-191865996
7 CATAGAAGCAATACTCTGTTGCC GGTTATCAAGGAAGAATTCAGAGTC 284bp chr2:191864226-191864509
8 TTTGACCTGTCACTAGGCAGC TTTCCTCTCTTCTAAACTTTGAGTCC 287bp chr2:191862862-191863148
9 GCCCCAGTTGAGAATGAAATG CAGGGTAAGCAGAAGCTGGAC 401bp chr2:191862439-191862839
10 ATTCCCTTTAATCCAGGCTGC TGAATTAACGGTAAAATGTTCCTC 298bp chr2:191859716-191860013
11 TTCAGGAAAATCATTGTGATTGC TTTCCTCAAAAGCACCCTATATAAC 268bp chr2:191855854-191856121
12 TAAGCGGTTCCACAGCTAGG GTGGAGGAATCTGTGCTTGAG 344bp chr2:191854262-191854605
13-14 TCATCATTCACTTACACTCTTATGCTC ACAGGAAGACACCAGCCAC 459bp chr2:191851440-191851898
15 GGTTGTGATTCCAAGAGATCC ACCAACTGACCTGTCCTTGC 347bp chr2:191850274-191850620
16 TGAGTCTTCAGACTTGCCACTG AATACCTCCAGAACAAACACTGAG 309bp chr2:191848940-191849248
17 AGGCTAAGCAGTTATCTGAAGGTG ATGCAGCAGTGAGAGCGTG 234bp chr2:191848303-191848536
18 CTCTTCCCCTACTGTGAAAGCA CTCATCATGTCAGGGACTTCCT 646bp chr2:191846734-191847379
19 CAAGAGCCATCCGTCCAAC GCCAGAGAAGAACACGCC 243bp chr2:191845227-191845469
20 CTGTGCTGAATGGGACAGTTC AATACTGAAGCTGGACTCAGGC 303bp chr2:191844415-191844717
21 ACATTCCAGCCATTTTCTTGG ATGTCCCAAACGCACTATCTG 338bp chr2:191843483-191843820
22 TGTCCTCTTTCATTTTGGGATG CTGCATGGGTGGAGTTTCAG 326bp chr2:191841483-191841808
23 TCCCCCCGAAAAGTTGATGT GTTCAGCTGTGATGGCGATAG 277bp chr2:191840413-191840689
24 TCTGGCTATTGCTTATCACATCC GCTCATCTGAGCACTGCACTC 482bp chr2:191839416-191839897
25a GACAAGCAATGTTTAATGTATGAGTC CCCACAGAAATTTACCGTTCC 523bp chr2:191835000-191835522

IV. Ethanol precipitation

V. Sequencing analysis