TNFRSF1A
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)
Genomic regions amplified by ePCR with
the primer
1 GGACTCCTGTGCTTGTGACTG GCAGTGGCTGAGGTTAGGAC 567bp chr12:6450792-6451358
2 TTCACTGAGGAAGGACTTGAGC ACAAGTAGGTTCCTGTGAATGG 496bp chr12:6443020-6443515
3 TAGAGACTTCAGGGAATCGGC CATAGACAGGCACCCACACAC 288bp chr12:6442841-6443128
4 GGACACTGCATGGATGTGAG CCAGAGAGGAGTTGGTTGTCAG 393bp chr12:6442406-6442798
5 CTGTCCCTCGTCACTTCCTC AGCTAATGGTTCCCACCAGTC 236bp chr12:6442159-6442394
6 TGTCCAACAATCTGTGTGGTTG GTAGAGCTTGGACTTCCACCG 403bp chr12:6439771-6440173
7 CAGGTGAGGAGAAGTGACCTG GGACGAGAGAGCTACTGATGC 443bp chr12:6439579-6440021
8 GAGGCATGTCACCACAAGTC GATCTGAGCATTAGGCAATTATAAGG 197bp chr12:6439363-6439559
9 GCTCGCCTCATTCCCTCTAAG TCTCCACCACGGCGTACAG 582bp chr12:6438753-6439334
10a TAGACAGTGAGTTTCTCCCGC GCATAGCGTCCCTCATCCTC 727bp chr12:6438223-6438949

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)
Genomic regions amplified by ePCR with
the primer
1 GGACTCCTGTGCTTGTGACTG GCAGTGGCTGAGGTTAGGAC 567bp chr12:6450792-6451358
2 TTCACTGAGGAAGGACTTGAGC ACAAGTAGGTTCCTGTGAATGG 496bp chr12:6443020-6443515
3 TAGAGACTTCAGGGAATCGGC CATAGACAGGCACCCACACAC 288bp chr12:6442841-6443128
4 GGACACTGCATGGATGTGAG CCAGAGAGGAGTTGGTTGTCAG 393bp chr12:6442406-6442798
5 CTGTCCCTCGTCACTTCCTC AGCTAATGGTTCCCACCAGTC 236bp chr12:6442159-6442394
6 TGTCCAACAATCTGTGTGGTTG GTAGAGCTTGGACTTCCACCG 403bp chr12:6439771-6440173
7 CAGGTGAGGAGAAGTGACCTG GGACGAGAGAGCTACTGATGC 443bp chr12:6439579-6440021
8 GAGGCATGTCACCACAAGTC GATCTGAGCATTAGGCAATTATAAGG 197bp chr12:6439363-6439559
9 GCTCGCCTCATTCCCTCTAAG TCTCCACCACGGCGTACAG 582bp chr12:6438753-6439334
10a TAGACAGTGAGTTTCTCCCGC GCATAGCGTCCCTCATCCTC 727bp chr12:6438223-6438949

IV. Ethanol precipitation

V. Sequencing analysis