FAS
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AAGCCTTTAGAAAGGGCAGGAGG GGCCTGAGGGAAGGTCCTAACAG 707bp chr10:90750145+90750851
2 CAGTGGAGCCCTCACATTGTC TGGAGCCAAATAGACCAGTCTTCTC 553bp chr10:90762658+90763210
3 CATCCCTCTATAGTTCCCACCCTG TGTGTGTCAACATAGCACCACAG 321bp chr10:90767383+90767703
4 CTGGTGTCATGCTGTGACTGTTG TAGTTGCTTGGAGGCAAAGCAGG 364bp chr10:90768518+90768881
7 GGCCAGCCTGTGGTAATTAGATG CACTTGAGCCCAGGACTTTGAG 515bp chr10:90771524+90772038
8 GCTTAGTTTCTGGCAAGGCCG GAAAAGTTTCTTAAGCCTCAGACTATTG 481bp chr10:90772888+90773368
5-6 ATACGTTTGCCAGAGATGCAAAG AAGAATGAGGCAAATCTTTGTGAAC 471bp chr10:90770176+90770646
9a TCTTGTTTCTGTATTCCCCTAGTCAGC AAACCAAGCAGTATTTACAGCCAGC 570bp chr10:90773725+90774294

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
1 AAGCCTTTAGAAAGGGCAGGAGG GGCCTGAGGGAAGGTCCTAACAG 707bp chr10:90750145+90750851
2 CAGTGGAGCCCTCACATTGTC TGGAGCCAAATAGACCAGTCTTCTC 553bp chr10:90762658+90763210
3 CATCCCTCTATAGTTCCCACCCTG TGTGTGTCAACATAGCACCACAG 321bp chr10:90767383+90767703
4 CTGGTGTCATGCTGTGACTGTTG TAGTTGCTTGGAGGCAAAGCAGG 364bp chr10:90768518+90768881
7 GGCCAGCCTGTGGTAATTAGATG CACTTGAGCCCAGGACTTTGAG 515bp chr10:90771524+90772038
8 GCTTAGTTTCTGGCAAGGCCG GAAAAGTTTCTTAAGCCTCAGACTATTG 481bp chr10:90772888+90773368
5-6 ATACGTTTGCCAGAGATGCAAAG AAGAATGAGGCAAATCTTTGTGAAC 471bp chr10:90770176+90770646
9a TCTTGTTTCTGTATTCCCCTAGTCAGC AAACCAAGCAGTATTTACAGCCAGC 570bp chr10:90773725+90774294

IV. Ethanol precipitation

V. Sequencing analysis