IRF8
Primary Info
Mutation
Mouse studies
DNA sequencing


Click on each title for more information
I. DNA amplification
Kit
GE:illustra GenomiPhi V2 DNA Amplification Kit

II. PCR
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 TGCAAACCTCTGAGTTTCCTG TCCACTAGTCCATGAGGGAAAG 336bp chr16:85936525+85936860
3 AAGATTCATGGGAAGGGAAGG CTGTGGTGAAGGCTACAGGC 352bp chr16:85942492+85942843
4 TTCCCAAACCAATGAAGACAC GTGGAGATGCCACTTCCTCAG 355bp chr16:85945043+85945397
5 TCTCCTGAGATAAAGGTGACAATATG CACCTCTCAGGGCTGAGCTAC 370bp chr16:85946611+85946980
6 TGAAACAATGGCTCTCTGCTC CCTAGACTTCCACACCAGGTC 348bp chr16:85947895+85948242
7 GTGAGACAGACTGTGCACTTGG TGCAAATGGCACACAGATAGG 538bp chr16:85951942+85952479
8 GCCTAAATGCTACAAGCCCTG CACAAGATCAAGACCATCCTGG 477bp chr16:85953606+85954082
9a TGGCTAGGGTCAAAGACAGTG GTCTTTGGAAATGATAGTTAACCG 574bp chr16:85954635+85955208

III. Sequencing reaction
Kit
BigDye® Terminator v3.1 Cycle Sequencing Kit
Primer details
Exon No. Forward sequence Reverse sequence Expected PCR
product size (bp)		 Genomic regions amplified by ePCR with
the primer
2 TGCAAACCTCTGAGTTTCCTG TCCACTAGTCCATGAGGGAAAG 336bp chr16:85936525+85936860
3 AAGATTCATGGGAAGGGAAGG CTGTGGTGAAGGCTACAGGC 352bp chr16:85942492+85942843
4 TTCCCAAACCAATGAAGACAC GTGGAGATGCCACTTCCTCAG 355bp chr16:85945043+85945397
5 TCTCCTGAGATAAAGGTGACAATATG CACCTCTCAGGGCTGAGCTAC 370bp chr16:85946611+85946980
6 TGAAACAATGGCTCTCTGCTC CCTAGACTTCCACACCAGGTC 348bp chr16:85947895+85948242
7 GTGAGACAGACTGTGCACTTGG TGCAAATGGCACACAGATAGG 538bp chr16:85951942+85952479
8 GCCTAAATGCTACAAGCCCTG AAGCTGCCCATACTTACCTGC 242bp chr16:85953606+85953847
9a TGGCTAGGGTCAAAGACAGTG GTCTTTGGAAATGATAGTTAACCG 574bp chr16:85954635+85955208

IV. Ethanol precipitation

V. Sequencing analysis